Improved Vectors for Selecting Resistance to Hygromycin
نویسندگان
چکیده
منابع مشابه
On Selecting Tests for Equality of Two Normal Mean Vectors.
The conventional approach for testing the equality of two normal mean vectors is to test first the equality of covariance matrices, and if the equality assumption is tenable, then use the two-sample Hotelling T (2) test. Otherwise one can use one of the approximate tests for the multivariate Behrens-Fisher problem. In this article, we study the properties of the Hotelling T (2) test, the conven...
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Incorporation into plasmids of genes conferring resistance to aminoglycoside antibiotics such as hygromycin B is currently utilized for selection in experiments involving gene transfer in eukaryotic cells. Using a subclone of Caco-2 cells stably transfected with an episomal plasmid containing the hygromycin resistance gene, we observed that transformed cells subcultured in the presence of hygro...
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Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus. Ribosomes from this Gram-positive mycelial bacterium are inhibited in vitro by the antibiotic. In contrast, the streptomycete is highly resistant to the drug in vivo since it possesses hygromycin B phosphotransferase activity. This enzyme has been shown by g...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1994
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1367